AT26-10 Chemosynthetic Microbial Communities at Deep-Sea VentsEntry ID: gov.noaa.ngdc.mgg.wcd.AT26-10_EM122
Abstract: The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9?46?N to 9?53?N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30?C), and a higher temperature vent (e.g., L-vent; T: ... 30-60?C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.
Purpose: Deep-sea hydrothermal vents, first discovered in 1977, are ?poster child? ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions ... in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, we are carrying out an interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, we will address the following objectives:
Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities.
Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents.
Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents.
Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.
Data Set Citation
Dataset Title: AT26-10 Chemosynthetic Microbial Communities at Deep-Sea Vents
Dataset Release Date: 2014-01-15
This data set description is a member of a collection. The collection is described in
Start Date: 2014-01-15Stop Date: 2014-01-20
OCEANS > OCEAN ACOUSTICS > ACOUSTIC SCATTERING
OCEANS > AQUATIC SCIENCES > FISHERIES
OCEANS > BATHYMETRY/SEAFLOOR TOPOGRAPHY > BATHYMETRY
BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS
BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS
BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC
BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC
OCEANS > BATHYMETRY/SEAFLOOR TOPOGRAPHY > SEAFLOOR TOPOGRAPHY
Use Constraints Resource may not be available for up to one year after initial collection.
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
Data Set Progress
Distribution Format: Kongsberg/Simrad instrument files (.raw or .wcd)
Role: DIF AUTHOR
Phone: (303) 497-4742
Email: wcd.info at noaa.gov
325 Broadway, Mail Code E/GC3
Province or State: CO
Postal Code: 80305-3328
Email: ssievert at who.edu
City: Woods Hole
Province or State: MA
Postal Code: 02543
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2015-10-01
Last DIF Revision Date: 2018-05-22